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ADD1 Antibody

Purified Mouse Monoclonal Antibody

  • E - ADD1 Antibody AO2026a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ADD1 Antibody AO2026a

    Figure 2:Western blot analysis using ADD1 mAb
    against human ADD1 (AA: 1-193)
    recombinant protein. (Expected MW is 47.8 kDa)

  • WB - ADD1 Antibody AO2026a

    Figure 3:Western blot analysis using ADD1 mAb
    against HEK293 (1) and ADD1 (AA: 1-193)-hIgGFc transfected HEK293 (2) cell lysate.

  • IHC - ADD1 Antibody AO2026a

    Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using 1-193 mouse mAb with DAB staining.

  • IHC - ADD1 Antibody AO2026a

    Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using 1-193 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35611
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5D4H1
Isotype IgG1
Calculated MW 81kDa
Description Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Immunogen Purified recombinant fragment of human ADD1 (AA: 1-193) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 118
Other Names Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADD1
Synonyms ADDA
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Cellular Location Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit
Research Areas
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EMBO J. 2012 Mar 21;31(6):1453-66.Traffic. 2011 Oct;12(10):1327-40.

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$ 385.00
Cat# AO2026a
(40 western blots)
Availability: 7-10 days
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