|Application ||WB, IHC, FC, ICC, E|
|Description||Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.|
|Immunogen||Purified recombinant fragment of human ADD1 (AA: 1-193) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA|
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ADD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side|
|Tissue Location||Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit|
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