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AIM2 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - AIM2 Antibody AO2057a

    Figure 1:Western blot analysis using AIM2 mAb against human AIM2 (AA: 1-195) recombinant protein. (Expected MW is 48.2 kDa)

  • E - AIM2 Antibody AO2057a

    Figure 2:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - AIM2 Antibody AO2057a

    Figure 3:Western blot analysis using AIM2 mAb against HEK293 (1) and AIM2 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - AIM2 Antibody AO2057a

    Figure 4:Immunofluorescence analysis of A549 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IF - AIM2 Antibody AO2057a

    Figure 5:Immunofluorescence analysis of MCF-7 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - AIM2 Antibody AO2057a

    Figure 6:Flow cytometric analysis of Hela cells using AIM2 mouse mAb (green) and negative control (red).

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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession O14862
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3C4G11
Isotype IgG1
Calculated MW 39kDa
Description AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
Immunogen Purified recombinant fragment of human AIM2 (AA: 1-195) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 9447
Other Names Interferon-inducible protein AIM2, Absent in melanoma 2, AIM2
Dilution WB~~1/500 - 1/2000
E~~1/10000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAIM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AIM2
Function Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
Cellular Location Nucleus. Cytoplasm. Note=Activated inflammasomes can aggregate in the cytosol as speck-like particles
Tissue Location Expressed in spleen, small intestine, peripheral blood leukocytes, and testis
Research Areas
Citations (0)

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References

1.Mol Cancer Res. 2013 Oct;11(10):1193-202.2.Int J Cancer. 2010 Apr 15;126(8):1838-49.

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$ 385.00
Cat# AO2057a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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