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TERT Antibody

Purified Mouse Monoclonal Antibody

  • E - TERT Antibody AO2069a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - TERT Antibody AO2069a

    Figure 2:Western blot analysis using TERT mAb against human TERT (AA: 1029-1132) recombinant protein. (Expected MW is 37.1 kDa)

  • WB - TERT Antibody AO2069a

    Figure 3:Western blot analysis using TERT mAb against HEK293 (1) and TERT (AA: 1029-1132)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - TERT Antibody AO2069a

    Figure 4:Flow cytometric analysis of Hela cells using TERT mouse mAb (green) and negative control (red).

  • IHC - TERT Antibody AO2069a

    Figure 5:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using TERT mouse mAb with DAB staining.

  • IHC - TERT Antibody AO2069a

    Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using TERT mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O14746
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3H2C12
Isotype IgG1
Calculated MW 127kDa
Description Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
Immunogen Purified recombinant fragment of human TERT (AA: 1029-1132) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7015
Other Names Telomerase reverse transcriptase,, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2, TERT, EST2, TCS1, TRT
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTERT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms EST2, TCS1, TRT
Function Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA- dependent extension of 3'-chromosomal termini with the 6- nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
Cellular Location Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Chromosome, telomere. Cytoplasm. Nucleus, PML body Note=Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress- mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT
Tissue Location Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T-lymphocytes, and at a low to undetectable level in peripheral blood T- lymphocytes.
Research Areas
Citations (0)

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1.Cancer Res. 2013 Dec 15;73(24):7162-7.2.PLoS One. 2013 Sep 25;8(9):e75253.

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$ 385.00
Cat# AO2069a
(40 western blots)
Availability: 7-10 days
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