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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAB11FIP1 Antibody   

RAB11FIP1 Antibody

Purified Mouse Monoclonal Antibody

  • E - RAB11FIP1 Antibody AO2084a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAB11FIP1 Antibody AO2084a

    Figure 2:Western blot analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)

  • WB - RAB11FIP1 Antibody AO2084a

    Figure 3:Western blot analysis using RAB11FIP1 mAb against HEK293 (1) and RAB11FIP1 (AA: 130-271)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - RAB11FIP1 Antibody AO2084a

    Figure 4:Western blot analysis using RAB11FIP1 mouse mAb against Raji (1), SW620 (2), A431 (3), SW480 (4), HepG2 (5), Hela (6), and NIH3T3 (7) cell lysate.

  • IF - RAB11FIP1 Antibody AO2084a

    Figure 5:Immunofluorescence analysis of Hela cells using RAB11FIP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IHC - RAB11FIP1 Antibody AO2084a

    Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RAB11FIP1 mouse mAb with DAB staining.

  • IHC - RAB11FIP1 Antibody AO2084a

    Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.

  • FC - RAB11FIP1 Antibody AO2084a

    Figure 8:Flow cytometric analysis of Raji cells using RAB11FIP1 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6WKZ4
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1A12B9D8
Isotype IgG1
Calculated MW 137.2kDa
Description This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome
Immunogen Purified recombinant fragment of human RAB11FIP1 (AA: 130-271) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 80223
Other Names Rab11 family-interacting protein 1, Rab11-FIP1, Rab-coupling protein, RAB11FIP1, RCP
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB11FIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB11FIP1
Synonyms RCP
Function A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.
Cellular Location Recycling endosome. Note=Rab11A rather than Rab4A mediates localization in the endocytic recycling compartment (ERC)
Tissue Location Isoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine
Research Areas
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1.J Clin Invest. 2009 Aug;119(8):2171-83.2.J Mol Endocrinol. 2005 Oct;35(2):357-72.

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$ 385.00
Cat# AO2084a
Availability: 7-10 days
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