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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAB11FIP1 Antibody   

RAB11FIP1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - RAB11FIP1 Antibody AO2084a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAB11FIP1 Antibody AO2084a

    Figure 2:Western blot analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)

  • WB - RAB11FIP1 Antibody AO2084a

    Figure 3:Western blot analysis using RAB11FIP1 mAb against HEK293 (1) and RAB11FIP1 (AA: 130-271)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - RAB11FIP1 Antibody AO2084a

    Figure 4:Western blot analysis using RAB11FIP1 mouse mAb against Raji (1), SW620 (2), A431 (3), SW480 (4), HepG2 (5), Hela (6), and NIH3T3 (7) cell lysate.

  • IF - RAB11FIP1 Antibody AO2084a

    Figure 5:Immunofluorescence analysis of Hela cells using RAB11FIP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IHC - RAB11FIP1 Antibody AO2084a

    Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RAB11FIP1 mouse mAb with DAB staining.

  • IHC - RAB11FIP1 Antibody AO2084a

    Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.

  • FC - RAB11FIP1 Antibody AO2084a

    Figure 8:Flow cytometric analysis of Raji cells using RAB11FIP1 mouse mAb (green) and negative control (red).

  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q6WKZ4
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1A12B9D8
Isotype IgG1
Calculated MW 137.2kDa
Description This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome
Immunogen Purified recombinant fragment of human RAB11FIP1 (AA: 130-271) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Other Names Rab11 family-interacting protein 1, Rab11-FIP1, Rab-coupling protein, RAB11FIP1, RCP
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB11FIP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB11FIP1
Synonyms RCP
Function A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.
Cellular Location Recycling endosome. Note=Rab11A rather than Rab4A mediates localization in the endocytic recycling compartment (ERC)
Tissue Location Isoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine EMBL; AF368294; AAM09571.1; -; mRNA EMBL; DQ236342; ABB43161.1; -; mRNA EMBL; AY280966; AAQ18784.1; -; mRNA EMBL; AY280968; AAQ18786.1; -; mRNA EMBL; AY280970; AAQ18788.1; -; mRNA EMBL; AK026275; BAB15424.1; -; mRNA EMBL; AK122583; BAC56924.1; -; mRNA EMBL; AK291781; BAF84470.1; -; mRNA EMBL; AC130304; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC077720; AAH77720.1; -; mRNA CCDS; CCDS34881.1; -. [Q6WKZ4-3] CCDS; CCDS34882.1; -. [Q6WKZ4-4] RefSeq; NP_001002814.2; NM_001002814.2. [Q6WKZ4-4] RefSeq; NP_079427.4; NM_025151.4. [Q6WKZ4-3] UniGene; Hs.191179; - PDB; 4D0G; X-ray; 2.50 A; C=1216-1283 PDBsum; 4D0G; - ProteinModelPortal; Q6WKZ4; - SMR; Q6WKZ4; - BioGrid; 123189; 31 CORUM; Q6WKZ4; - IntAct; Q6WKZ4; 18 MINT; Q6WKZ4; - STRING; 9606.ENSP00000331342; - iPTMnet; Q6WKZ4; - PhosphoSitePlus; Q6WKZ4; - BioMuta; RAB11FIP1; - DMDM; 519668675; - EPD; Q6WKZ4; - PaxDb; Q6WKZ4; - PeptideAtlas; Q6WKZ4; - PRIDE; Q6WKZ4; - ProteomicsDB; 67762; - ProteomicsDB; 67763; -. [Q6WKZ4-1] ProteomicsDB; 67764; -. [Q6WKZ4-2] ProteomicsDB; 67765; -. [Q6WKZ4-3] ProteomicsDB; 67766; -. [Q6WKZ4-5] Ensembl; ENST00000287263; ENSP00000287263; ENSG00000156675. [Q6WKZ4-3] Ensembl; ENST00000330843; ENSP00000331342; ENSG00000156675. [Q6WKZ4-4] Ensembl; ENST00000524118; ENSP00000430680; ENSG00000156675. [Q6WKZ4-2] GeneID; 80223; - KEGG; hsa:80223; - UCSC; uc003xkm.3; human. [Q6WKZ4-4] CTD; 80223; - DisGeNET; 80223; - EuPathDB; HostDB:ENSG00000156675.15; - GeneCards; RAB11FIP1; - HGNC; HGNC:30265; RAB11FIP1 HPA; CAB017037; - HPA; HPA023904; - HPA; HPA024010; - HPA; HPA025960; - MIM; 608737; gene neXtProt; NX_Q6WKZ4; - OpenTargets; ENSG00000156675; - PharmGKB; PA134937501; - eggNOG; ENOG410IGQZ; Eukaryota eggNOG; ENOG410Z6B0; LUCA GeneTree; ENSGT00530000063203; - HOGENOM; HOG000234700; - InParanoid; Q6WKZ4; - KO; K12484; - OMA; YQSKASD; - OrthoDB; EOG091G09IV; - PhylomeDB; Q6WKZ4; - TreeFam; TF326172; - ChiTaRS; RAB11FIP1; human GeneWiki; RAB11FIP1; - GenomeRNAi; 80223; - PRO; PR:Q6WKZ4; - Proteomes; UP000005640; Chromosome 8 Bgee; ENSG00000156675; - CleanEx; HS_RAB11FIP1; - ExpressionAtlas; Q6WKZ4; baseline and differential Genevisible; Q6WKZ4; HS GO; GO:0005737; C:cytoplasm; IBA:GO_Central GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell GO; GO:0055037; C:recycling endosome; IEA:UniProtKB-SubCell GO; GO:0017137; F:Rab GTPase binding; IBA:GO_Central GO; GO:0070164; P:negative regulation of adiponectin secretion; IDA:CACAO GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW GO; GO:0045055; P:regulated exocytosis; IBA:GO_Central Gene3D; 2.60.40.150; -; 1 InterPro; IPR000008; C2_dom InterPro; IPR035892; C2_domain_sf InterPro; IPR037245; FIP-RBD_C_sf InterPro; IPR037789; FIP_classI InterPro; IPR019018; Rab-bd_FIP-RBD PANTHER; PTHR15746; PTHR15746; 1 Pfam; PF00168; C2; 1 Pfam; PF09457; RBD-FIP; 1 SMART; SM00239; C2; 1 SUPFAM; SSF144270; SSF144270; 1 PROSITE; PS50004; C2; 1 PROSITE; PS51511; FIP_RBD; 1 1: Evidence at protein level; 3D-structure; Alternative splicing; Complete proteome; Cytoplasmic vesicle; Endosome; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport CHAIN 1 1283 Rab11 family-interacting protein 1 /FTId=PRO_0000097304 DOMAIN 5 108 C2. {ECO:0000255|PROSITE- ProRule:PRU00041} DOMAIN 1211 1273 FIP-RBD. {ECO:0000255|PROSITE- ProRule:PRU00844} REGION 1219 1283 Necessary for interaction with RAB4A and RAB11A, subcellular location and endosomal recycling COMPBIAS 209 218 Poly-Lys COMPBIAS 556 609 Ser-rich MOD_RES 184 184 Phosphoserine {ECO:0000250|UniProtKB:Q9D620} MOD_RES 202 202 Phosphoserine MOD_RES 206 206 Phosphoserine MOD_RES 234 234 Phosphoserine MOD_RES 300 300 Phosphoserine MOD_RES 315 315 Phosphoserine MOD_RES 339 339 Phosphoserine MOD_RES 341 341 Phosphoserine {ECO:0000250|UniProtKB:Q9D620} MOD_RES 343 343 Phosphoserine MOD_RES 345 345 Phosphoserine MOD_RES 356 356 Phosphoserine MOD_RES 357 357 Phosphoserine MOD_RES 382 382 Phosphoserine {ECO:0000250|UniProtKB:Q9D620} MOD_RES 435 435 Phosphoserine MOD_RES 477 477 Phosphoserine MOD_RES 529 529 Phosphoserine MOD_RES 545 545 Phosphoserine MOD_RES 758 758 Phosphoserine MOD_RES 1135 1135 Phosphoserine VAR_SEQ 1 671 Missing (in isoform 3) /FTId=VSP_013726 VAR_SEQ 1 148 Missing (in isoform 4) /FTId=VSP_013727 VAR_SEQ 116 142 RDQGRRKTQWYKLKSKPGKKDKERGEI -> SSLGKSFFKT LKKRAWAIFLRLCLKKN (in isoform 5) /FTId=VSP_013728 VAR_SEQ 143 1283 Missing (in isoform 5) /FTId=VSP_013729 VAR_SEQ 541 1174 Missing (in isoform 2 and isoform 4) /FTId=VSP_013730 VAR_SEQ 1212 1221 KYSPSDPAFA -> VCPLRSWCVR (in isoform 4) /FTId=VSP_013731 VAR_SEQ 1222 1283 Missing (in isoform 4) /FTId=VSP_013732 VARIANT 622 622 Q -> K (in dbSNP:rs7341564) /FTId=VAR_069365 VARIANT 651 651 A -> V (in dbSNP:rs12541651) /FTId=VAR_059714 VARIANT 768 768 A -> V (in dbSNP:rs16887092) /FTId=VAR_056977 VARIANT 1185 1185 M -> T (in dbSNP:rs7817179) /FTId=VAR_022447 MUTAGEN 1254 1254 Y->F: Does not abolish the interaction with RAB11A, homooligomerization and subcellular location. Reduces the interaction with RAB4A MUTAGEN 1255 1255 I->E: Abolishes the interaction with RAB11A and RAB4A, homooligomerization and subcellular location MUTAGEN 1256 1256 D->N: Does not abolish the interaction with RAB11A, homooligomerization and subcellular location. Reduces the interaction with RAB4A CONFLICT 330 332 EAK -> QPT (in Ref. 1; AAM09571) CONFLICT 353 353 H -> T (in Ref. 1; AAM09571) CONFLICT 377 377 S -> T (in Ref. 1; AAM09571) CONFLICT 380 381 QL -> DV (in Ref. 1; AAM09571) CONFLICT 397 397 S -> T (in Ref. 1; AAM09571) CONFLICT 404 404 V -> I (in Ref. 1; AAM09571) CONFLICT 411 411 N -> K (in Ref. 1; AAM09571) CONFLICT 455 455 P -> L (in Ref. 1; AAM09571) CONFLICT 482 482 D -> G (in Ref. 2; AAQ18788) HELIX 1233 1265 {ECO:0000244|PDB:4D0G} HELIX 1267 1270 {ECO:0000244|PDB:4D0G} SEQUENCE 1283 AA; 137167 MW; 19DF0C848435780B CRC64; MSLMVSAGRG LGAVWSPTHV QVTVLQARGL RAKGPGGTSD AYAVIQVGKE KYATSVSERS LGAPVWREEA TFELPSLLSS GPAAAATLQL TVLHRALLGL DKFLGRAEVD LRDLHRDQGR RKTQWYKLKS KPGKKDKERG EIEVDIQFMR NNMTASMFDL SMKDKSRNPF GKLKDKIKGK NKDSGSDTAS AIIPSTTPSV DSDDESVVKD KKKKSKIKTL LSKSNLQKTP LSQSMSVLPT SKPEKVLLRP GDFQSQWDED DNEDESSSAS DVMSHKRTAS TDLKQLNQVN FTLPKKEGLS FLGGLRSKND VLSRSNVCIN GNHVYLEQPE AKGEIKDSSP SSSPSPKGFR KKHLFSSTEN LAAGSWKEPA EGGGLSSDRQ LSESSTKDSL KSMTLPSYRP APLVSGDLRE NMAPANSEAT KEAKESKKPE SRRSSLLSLM TGKKDVAKGS EGENPLTVPG REKEGMLMGV KPGEDASGPA EDLVRRSEKD TAAVVSRQGS SLNLFEDVQI TEPEAEPESK SEPRPPISSP RAPQTRAVKP RLEVSPEAQP TARLPSPTDS PSSLPPLPSS SGQASVPSEL GHGADTQSSE SPSVFSSLSS PIAAPISTST PIESWPLVDR GQAKSEGPPL LPKAELQTES LTPVPNSGSS ALGSLFKQPS FPANKGTEDS LMGRTRETGT EKNTSSLELE ESLPEQPETG RQEEELPRFP CKKQDYSPSS GEAQEVPFAL SLSSDGAVSP VGELAAGGDR DLESQAGSLV ESKARDAAEE VAPPLPMGAS VPSIDSMMRK LEEMGLNLRK DQKKTKKRVS FSEQLFTEEA VAGAALLVEG HSSCPQELNP AWSVAGNASD GEPPESPHAE DSERESVTTP GPATCGAPAS PADHLLLPSQ EESFSEVPMS EASSAKDTPL FRMEGEDALV TQYQSKASDH EGLLSDPLSD LQLVSDFKSP IMADLNLSLP SIPEVASDDE RIDQVEDDGD QVEDDGETAK SSTLDIGALS LGLVVPCPER GKGPSGEADR LVLGEGLCDF RLQAPQASVT APSEQTTEFG IHKPHLGKSS SLDKQLPGPS GGEEEKPMGN GSPSPPPGTS LDNPVPSPSP SEIFPVTHSF PSSAHSDTHH TSTAESQKKA TAEGSAGRVE NFGKRKPLLQ AWVSPSETHP VSAQPGAGTG SAKHRLHPVK PMNAMATKVA NCSLGTATII SENLNNEVMM KKYSPSDPAF AYAQLTHDEL IQLVLKQKET ISKKEFQVRE LEDYIDNLLV RVMEETPNIL RIPTQVGKKA GKM
Research Areas
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References

1.J Clin Invest. 2009 Aug;119(8):2171-83.2.J Mol Endocrinol. 2005 Oct;35(2):357-72.

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Cat# AO2084a
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