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GLI1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - GLI1 Antibody AO2089a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - GLI1 Antibody AO2089a

    Figure 2:Western blot analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)

  • WB - GLI1 Antibody AO2089a

    Figure 3:Western blot analysis using GLI1 mAb against HEK293 (1) and GLI1 (AA: 284-449)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - GLI1 Antibody AO2089a

    Figure 4:Immunofluorescence analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - GLI1 Antibody AO2089a

    Figure 5:Flow cytometric analysis of HepG2 cells using GLI1 mouse mAb (green) and negative control (red).

  • IHC - GLI1 Antibody AO2089a

    Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GLI1 mouse mAb with DAB staining.

  • IHC - GLI1 Antibody AO2089a

    Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GLI1 mouse mAb with DAB staining.

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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P08151
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1B9F8
Isotype IgG1
Calculated MW 118kDa
Description This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human GLI1 (AA: 284-449) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2735
Other Names Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI, GLI1, GLI
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLI1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLI1
Synonyms GLI
Function Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'- GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). May regulate the transcription of specific genes during normal development (PubMed:19706761). May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761). Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable).
Cellular Location Cytoplasm. Nucleus. Note=Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441)
Tissue Location Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)
Research Areas
Citations (0)

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References

1.Mol Cancer. 2014 Jun 3;13:137.2.J Hematol Oncol. 2014 Mar 30;7:28.

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$ 385.00
Cat# AO2089a
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