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MGMT Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, ICC, E |
|---|---|
| Primary Accession | P16455 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1A11F8 |
| Isotype | IgG1 |
| Calculated MW | 21.6kDa |
| Description | MGMT (O-6-methylguanine-DNA methyltransferase) is a protein-coding gene. Diseases associated with MGMT include oligoastrocytoma, and spinal cord astrocytoma. GO annotations related to this gene include DNA-methyltransferase activity and calcium ion binding. |
| Immunogen | Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 4255 |
|---|---|
| Other Names | Methylated-DNA--protein-cysteine methyltransferase, 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT |
| Dilution | E~~1/10000 WB~~1/500 - 1/2000 IF~~1/200 - 1/1000 FC~~1/200 - 1/400 IHC~~1/200 - 1/1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MGMT |
|---|---|
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Cellular Location | Nucleus. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
1.Neuropathology. 2014 Jun;34(3):268-76.2.Clin Cancer Res. 2013 Apr 15;19(8):2265-72.
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