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MGMT Antibody

Purified Mouse Monoclonal Antibody

  • E - MGMT Antibody AO2095a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - MGMT Antibody AO2095a

    Figure 2:Western blot analysis using MGMT mAb against human MGMT (AA: 32-210) recombinant protein. (Expected MW is 45.2 kDa)

  • WB - MGMT Antibody AO2095a

    Figure 3:Western blot analysis using MGMT mAb against HEK293 (1) and MGMT (AA: 32-210)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - MGMT Antibody AO2095a

    Figure 4:Western blot analysis using MGMT mouse mAb against MCF-7 (1), Jurkat (2), HepG2 (3), and PC-3 (4) cell lysate.

  • IF - MGMT Antibody AO2095a

    Figure 5:Immunofluorescence analysis of Hela cells using MGMT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - MGMT Antibody AO2095a

    Figure 6:Flow cytometric analysis of Hela cells using MGMT mouse mAb (green) and negative control (red).

  • IHC - MGMT Antibody AO2095a

    Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MGMT mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16455
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1A11F8
Isotype IgG1
Calculated MW 21.6kDa
Description MGMT (O-6-methylguanine-DNA methyltransferase) is a protein-coding gene. Diseases associated with MGMT include oligoastrocytoma, and spinal cord astrocytoma. GO annotations related to this gene include DNA-methyltransferase activity and calcium ion binding.
Immunogen Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4255
Other Names Methylated-DNA--protein-cysteine methyltransferase,, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Cellular Location Nucleus.
Citations (0)

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1.Neuropathology. 2014 Jun;34(3):268-76.2.Clin Cancer Res. 2013 Apr 15;19(8):2265-72.

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$ 385.00
Cat# AO2095a
(40 western blots)
Availability: 7-10 days
Bulk Size
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