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Notch3 Antibody

Purified Mouse Monoclonal Antibody

  • E - Notch3 Antibody AO2162a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - Notch3 Antibody AO2162a

    Figure 2:Western blot analysis using Notch3 mAb against human Notch3 (AA: 47-156) recombinant protein. (Expected MW is 37.3 kDa)

  • WB - Notch3 Antibody AO2162a

    Figure 3:Western blot analysis using Notch3 mAb against HEK293 (1) and Notch3 (AA: 47-156)-hIgGFc transfected HEK293 (2) cell lysate.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UM47
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E2D9
Isotype IgG2a
Calculated MW 243.6kDa
Description This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Immunogen Purified recombinant fragment of human Notch3 (AA: 47-156) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4854
Other Names Neurogenic locus notch homolog protein 3, Notch 3, Notch 3 extracellular truncation, Notch 3 intracellular domain, NOTCH3
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNotch3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Ubiquitously expressed in fetal and adult tissues
Research Areas
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1.Cancer Res. 2014 Jun 15;74(12):3282-93.2.Int J Cancer. 2013 Dec 1;133(11):2577-86.

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$ 385.00
Cat# AO2162a
(40 western blots)
Availability: 7-10 days
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