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KPNA2 Antibody

Purified Mouse Monoclonal Antibody

  • E - KPNA2 Antibody AO2205a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - KPNA2 Antibody AO2205a

    Figure 2:Western blot analysis using KPNA2 mAb against human KPNA2 (AA: 1-530) recombinant protein. (Expected MW is 60.3 kDa)

  • WB - KPNA2 Antibody AO2205a

    Figure 3:Western blot analysis using KPNA2 mAb against HEK293 (1) and KPNA2 (AA: 1-530)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - KPNA2 Antibody AO2205a

    Figure 4:Western blot analysis using KPNA2 mouse mAb against Hela (1), HEK293 (2), and NIH/3T3 (3) cell lysate.

  • IF - KPNA2 Antibody AO2205a

    Figure 5:Immunofluorescence analysis of Hela cells using KPNA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - KPNA2 Antibody AO2205a

    Figure 6:Flow cytometric analysis of Hela cells using KPNA2 mouse mAb (green) and negative control (red).

  • IHC - KPNA2 Antibody AO2205a

    Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using KPNA2 mouse mAb with DAB staining.

  • IHC - KPNA2 Antibody AO2205a

    Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KPNA2 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P52292
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1E8B7
Isotype IgG1
Calculated MW 58kDa
Description The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.
Immunogen Purified recombinant fragment of human KPNA2 (AA: 1-530) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3838
Other Names Importin subunit alpha-1, Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha, KPNA2, RCH1, SRP1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKPNA2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KPNA2
Synonyms RCH1, SRP1
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed ubiquitously.
Research Areas
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1.Mod Pathol. 2014 Jan;27(1):96-106.2.PLoS One. 2013;8(3):e57911.

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$ 385.00
Cat# AO2205a
(40 western blots)
Availability: 7-10 days
Bulk Size
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