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TH Antibody

Purified Mouse Monoclonal Antibody

  • E - TH Antibody AO2215a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - TH Antibody AO2215a

    Figure 2:Western blot analysis using TH mAb against human TH (AA: 44-208) recombinant protein. (Expected MW is 44 kDa)

  • WB - TH Antibody AO2215a

    Figure 3:Western blot analysis using TH mAb against HEK293 (1) and TH (AA: 44-208)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - TH Antibody AO2215a

    Figure 4:Western blot analysis using TH mouse mAb against SH-SY5Y (1) and PC-12 (2) cell lysate.

  • IF - TH Antibody AO2215a

    Figure 5:Immunofluorescence analysis of Hela cells using TH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - TH Antibody AO2215a

    Figure 6:Flow cytometric analysis of Hela cells using TH mouse mAb (green) and negative control (red).

  • FC - TH Antibody AO2215a

    Figure 7:Flow cytometric analysis of MCF-7 cells using TH mouse mAb (green) and negative control (red).

  • IHC - TH Antibody AO2215a

    Figure 8:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TH mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07101
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 1B8D2
Isotype IgG1
Calculated MW 58.6kDa
Description The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Immunogen Purified recombinant fragment of human TH (AA: 44-208) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7054
Other Names Tyrosine 3-monooxygenase,, Tyrosine 3-hydroxylase, TH, TH, TYH
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TH
Synonyms TYH
Function Plays an important role in the physiology of adrenergic neurons.
Tissue Location Mainly expressed in the brain and adrenal glands
Research Areas
Citations (0)

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1.Neurosci Lett. 2014 Jan 24;559:39-43.2.Biochem Biophys Res Commun. 2011 Nov 4;414(4):712-8.

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$ 385.00
Cat# AO2215a
(40 western blots)
Availability: 7-10 days
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