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TBCC Antibody

Purified Mouse Monoclonal Antibody

  • E - TBCC Antibody AO2219a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - TBCC Antibody AO2219a

    Figure 2:Western blot analysis using TBCC mAb against human TBCC (AA: 1-196) recombinant protein. (Expected MW is 47.9 kDa)

  • WB - TBCC Antibody AO2219a

    Figure 3:Western blot analysis using TBCC mAb against HEK293 (1) and TBCC (AA: 1-196)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - TBCC Antibody AO2219a

    Figure 4:Immunofluorescence analysis of Hela cells using TBCC mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IHC - TBCC Antibody AO2219a

    Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TBCC mouse mAb with DAB staining.

  • IHC - TBCC Antibody AO2219a

    Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TBCC mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15814
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7G6H1
Isotype IgG1
Calculated MW 39.2kDa
Description Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.
Immunogen Purified recombinant fragment of human *** (AA: 1-196) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6903
Other Names Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, TBCC
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBCC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Tubulin-folding protein; involved in the final step of the tubulin folding pathway.
Cellular Location Cytoplasm. Note=Detected predominantly in the photoreceptor connecting cilium
Tissue Location Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level).
Research Areas
Citations (0)

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1.PLoS One. 2011;6(10):e25912.2.BMC Cancer. 2010 Apr 12;10:135.

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$ 385.00
Cat# AO2219a
(40 western blots)
Availability: 7-10 days
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