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RAD21 Antibody

Purified Mouse Monoclonal Antibody

  • E - RAD21 Antibody AO2226a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - RAD21 Antibody AO2226a

    Figure 2:Western blot analysis using RAD21 mAb against human RAD21 (AA: 287-403) recombinant protein. (Expected MW is 39.3 kDa)

  • WB - RAD21 Antibody AO2226a

    Figure 3:Western blot analysis using RAD21 mAb against HEK293 (1) and RAD21 (AA: 287-403)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - RAD21 Antibody AO2226a

    Figure 4:Western blot analysis using RAD21 mouse mAb against Hela (1), HEK293 (2), K562 (3), C6 (4), *** (5), and COS7 (6) cell lysate.

  • IF - RAD21 Antibody AO2226a

    Figure 5:Immunofluorescence analysis of Hela cells using RAD21 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - RAD21 Antibody AO2226a

    Figure 6:Flow cytometric analysis of Hela cells using RAD21 mouse mAb (green) and negative control (red).

  • IHC - RAD21 Antibody AO2226a

    Figure 7:Immunohistochemical analysis of paraffin-embedded brain tissues using RAD21 mouse mAb with DAB staining.

  • IHC - RAD21 Antibody AO2226a

    Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using RAD21 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60216
Reactivity Human, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 1B6D1
Isotype IgG1
Calculated MW 71.7kDa
Description The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Immunogen Purified recombinant fragment of human RAD21 (AA: 287-403) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5885
Other Names Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog, RAD21, HR21, KIAA0078, NXP1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD21
Synonyms HR21, KIAA0078, NXP1
Function Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
Cellular Location Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis
Research Areas
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1.Breast Cancer Res. 2012 Apr 26;14(2):R69.2.Breast Cancer Res. 2011 Jan 21;13(1):R9.

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$ 385.00
Cat# AO2226a
(40 western blots)
Availability: 7-10 days
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