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CFHR5 Antibody

Purified Mouse Monoclonal Antibody

  • E - CFHR5 Antibody AO2242a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - CFHR5 Antibody AO2242a

    Figure 2:Western blot analysis using CFHR5 mAb against human CFHR5 (AA: 344-569) recombinant protein. (Expected MW is 51.8 kDa)

  • WB - CFHR5 Antibody AO2242a

    Figure 3:Western blot analysis using CFHR5 mAb against HEK293 (1) and CFHR5 (AA: 344-569)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - CFHR5 Antibody AO2242a

    Figure 4:Western blot analysis using CFHR5 mouse mAb against HepG2 (1), K562 (2), L-02 (3), SK-Hep-1 (4), SMMC-7721 (5), and NIH/3T3 (6) cell lysate.

  • IF - CFHR5 Antibody AO2242a

    Figure 5:Immunofluorescence analysis of Hela cells using CFHR5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - CFHR5 Antibody AO2242a

    Figure 6:Flow cytometric analysis of Hela cells using CFHR5 mouse mAb (green) and negative control (red).

  • IHC - CFHR5 Antibody AO2242a

    Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CFHR5 mouse mAb with DAB staining.

  • IHC - CFHR5 Antibody AO2242a

    Figure 8:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CFHR5 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BXR6
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 3A10A5
Isotype IgG2a
Calculated MW 64.4kDa
Description This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).
Immunogen Purified recombinant fragment of human CFHR5 (AA: 344-569) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 81494
Other Names Complement factor H-related protein 5, FHR-5, CFHR5, CFHL5, FHR5
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCFHR5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CFHR5
Synonyms CFHL5, FHR5
Function Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
Cellular Location Secreted.
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas
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1.Kidney Int. 2014 Apr;85(4):933-7. 2.Dis Model Mech. 2011 Nov;4(6):721-6.

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$ 385.00
Cat# AO2242a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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