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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CDH5 Antibody   

CDH5 Antibody

Purified Mouse Monoclonal Antibody

  • E - CDH5 Antibody AO2249a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CDH5 Antibody AO2249a
    Figure 1: Western blot analysis using CDH5 mAb against human CDH5 recombinant protein. (Expected MW is 47.6 kDa)
  • WB - CDH5 Antibody AO2249a
    Figure 2: Western blot analysis using CDH5 mouse mAb against MCF-7 (1), A549 (2), HUVE-12 (3) cell lysate, and rat lung (4) tissue lysate.
  • FC - CDH5 Antibody AO2249a
    Figure 3: Flow cytometric analysis of Jurkat cells using CDH5 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33151
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 3D5C7
Isotype IgG1
Calculated MW 87.5kDa
Description This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
Immunogen Purified recombinant fragment of human CDH5 (AA: 29-223 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1003
Other Names Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144, CDH5
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDH5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDH5
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Cellular Location Cell junction. Cell membrane; Single-pass type I membrane protein. Note=Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions
Tissue Location Endothelial tissues and brain.
Research Areas
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1.Blood. 2011 Feb 24;117(8):2515-26. 2.J Virol. 2010 Nov;84(21):11227-34.

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$ 385.00
Cat# AO2249a
(40 western blots)
Availability: 7-10 days
Bulk Size
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