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DCTN4 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - DCTN4 Antibody AO2290a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - DCTN4 Antibody AO2290a
    Figure 1: Western blot analysis using DCTN4 mAb against human DCTN4 recombinant protein. (Expected MW is 53.2 kDa)
  • WB - DCTN4 Antibody AO2290a
    Figure 2: Western blot analysis using DCTN4 mAb against HEK293 (1) and DCTN4 (AA: 57-298)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - DCTN4 Antibody AO2290a
    Figure 3: Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.
  • IF - DCTN4 Antibody AO2290a
    Figure 4: Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - DCTN4 Antibody AO2290a
    Figure 5: Flow cytometric analysis of HEK293 cells using DCTN4 mouse mAb (green) and negative control (red).
  • IHC - DCTN4 Antibody AO2290a
    Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DCTN4 mouse mAb with DAB staining.
  • IHC - DCTN4 Antibody AO2290a
    Figure 7: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DCTN4 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q9UJW0
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 3G9D7
Isotype IgG1
Calculated MW 52.3kDa
Description Dynactin 4 could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.The dynactin complex binds cargo, such as vesicles and organelles, to cytoplasmic dynein for retrograde microtubule-mediated trafficking and could feasibly be involved in the copper-regulated trafficking of ATP7B.
Immunogen Purified recombinant fragment of human DCTN4 (AA: 57-298) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 51164
Other Names Dynactin subunit 4, Dyn4, Dynactin subunit p62, DCTN4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDCTN4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DCTN4
Function Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=In skeletal muscles, demonstrates a punctate distribution along costameres (By similarity). Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the Golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels.
Research Areas
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References

Cell. 2008 Dec 26;135(7):1189-200. J Biol Chem. 2006 May 19;281(20):14006-14.

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$ 385.00
Cat# AO2290a
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