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PIWIL4 Antibody

Purified Mouse Monoclonal Antibody

  • E - PIWIL4 Antibody AO2292a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PIWIL4 Antibody AO2292a
    Figure 1: Western blot analysis using PIWIL4 mAb against human PIWIL4 recombinant protein. (Expected MW is 40.8 kDa)
  • WB - PIWIL4 Antibody AO2292a
    Figure 2: Western blot analysis using PIWIL4 mAb against HEK293 (1) and PIWIL4 (AA: 304-434)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - PIWIL4 Antibody AO2292a
    Figure 3: Western blot analysis using PIWIL4 mouse mAb against PC-3 (1) and PANC-1 (2) cell lysate.
  • FC - PIWIL4 Antibody AO2292a
    Figure 4: Flow cytometric analysis of HeLa cells using PIWIL4 mouse mAb (green) and negative control (red).
  • IHC - PIWIL4 Antibody AO2292a
    Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PIWIL4 mouse mAb with DAB staining.
  • IHC - PIWIL4 Antibody AO2292a
    Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z3Z4
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 10G9B11
Isotype IgG1
Calculated MW 96.6kDa
Description PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells
Immunogen Purified recombinant fragment of human PIWIL4 (AA: 304-434) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 143689
Other Names Piwi-like protein 4, PIWIL4, HIWI2, PIWI
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIWIL4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms HIWI2, PIWI
Function Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
Cellular Location Nucleus. Cytoplasm. Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity).
Tissue Location Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed
Research Areas
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1.FEBS Lett. 2012 May 7;586(9):1356-62. 2.Hum Reprod. 2010 Dec;25(12):2955-61.

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$ 385.00
Cat# AO2292a
(40 western blots)
Availability: 7-10 days
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