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CD33 Antibody

Purified Mouse Monoclonal Antibody

  • E - CD33 Antibody AO2309a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CD33 Antibody AO2309a
    Figure 1: Western blot analysis using DNAL4 mAb against human DNAL4 recombinant protein. (Expected MW is 44.7 kDa)
  • WB - CD33 Antibody AO2309a
    Figure 2: Western blot analysis using DNAL4 mAb against HEK293 (1) and DNAL4 (AA: 1-105)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - CD33 Antibody AO2309a
    Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DNAL4 mouse mAb with DAB staining.
  • IHC - CD33 Antibody AO2309a
    Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using DNAL4 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20138
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2C6B7
Isotype IgG1
Calculated MW 12kDa
Description DNAL4 is a component of the dynein motor complex
Immunogen Purified recombinant fragment of human DNAL4 (AA: 1-105) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 945
Other Names Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33, CD33, SIGLEC3
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD33 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD33
Synonyms SIGLEC3
Function Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Monocytic/myeloid lineage cells.
Research Areas
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1. J Neurosci. 2001 Feb 1;21(3):RC125.

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$ 385.00
Cat# AO2309a
(40 western blots)
Availability: 7-10 days
Bulk Size
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