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ALPI Antibody

Purified Mouse Monoclonal Antibody

  • E - ALPI Antibody AO2312a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ALPI Antibody AO2312a
    Figure 1: Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)
  • WB - ALPI Antibody AO2312a
    Figure 2: Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - ALPI Antibody AO2312a
    Figure 3: Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.
  • FC - ALPI Antibody AO2312a
    Figure 5: Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09923
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3B10E7
Isotype IgG1
Calculated MW 56.8kDa
Description There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Immunogen Purified recombinant fragment of human ALPI (AA: 397-458) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 248
Other Names Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase,, ALPI
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALPI Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Research Areas
Citations (0)

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1. Virchows Arch. 2012 Feb;460(2):157-61. 2. World J Gastroenterol. 2012 Jul 7;18(25):3254-9.

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$ 385.00
Cat# AO2312a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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