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ALPI Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ALPI Antibody AO2312a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - ALPI Antibody AO2312a
    Figure 1: Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)
    detail
  • WB - ALPI Antibody AO2312a
    Figure 2: Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - ALPI Antibody AO2312a
    Figure 3: Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.
    detail
  • FC - ALPI Antibody AO2312a
    Figure 5: Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P09923
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3B10E7
Isotype IgG1
Calculated MW 56.8kDa
Description There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Immunogen Purified recombinant fragment of human ALPI (AA: 397-458) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 248
Other Names Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, 3.1.3.1, ALPI
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALPI Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALPI
Function Alkaline phosphatase that can hydrolyze various phosphate compounds.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P15693}; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:P15693}
Research Areas
Citations (0)
citation

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References

1. Virchows Arch. 2012 Feb;460(2):157-61. 2. World J Gastroenterol. 2012 Jul 7;18(25):3254-9.

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$ 385.00
Cat# AO2312a
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