|Application ||WB, IHC, FC, E|
|Description||Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.|
|Immunogen||Purified recombinant fragment of human MRPL42 (AA: 10-142) expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||39S ribosomal protein L42, mitochondrial, L42mt, MRP-L42, 28S ribosomal protein S32, mitochondrial, MRP-S32, S32mt, 39S ribosomal protein L31, mitochondrial, L31mt, MRP-L31, MRPL42, MRPL31, MRPS32, RPML31|
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MRPL42 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MRPL31, MRPS32, RPML31|
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Provided below are standard protocols that you may find useful for product applications.
1. Genomics. 2003 May;81(5):468-80. 2. J Biol Chem. 2001 Nov 23;276(47):43958-69.
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