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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to TRAF2   

Mouse Monoclonal Antibody to TRAF2

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to TRAF2 AO2340a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to TRAF2 AO2340a
    Western blot analysis using TRAF2 mAb against human TRAF2 (AA: 39-188) recombinant protein. (Expected MW is 42.5 kDa)
  • WB - Mouse Monoclonal Antibody to TRAF2 AO2340a
    Western blot analysis using TRAF2 mAb against HEK293 (1) and TRAF2 (AA: 39-188)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to TRAF2 AO2340a
    Flow cytometric analysis of Hela cells using TRAF2 mouse mAb (green) and negative control (red).
  • FC - Mouse Monoclonal Antibody to TRAF2 AO2340a
    Flow cytometric analysis of HepG2 cells using TRAF2 mouse mAb (green) and negative control (red).
  • ICC - Mouse Monoclonal Antibody to TRAF2 AO2340a
    Immunofluorescence analysis of HL-7702 cells using TRAF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • ICC - Mouse Monoclonal Antibody to TRAF2 AO2340a
    Immunofluorescence analysis of MCF-7 cells using TRAF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q12933
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C2C3
Isotype Mouse IgG1
Calculated MW 55.8KD
Description The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.;
Immunogen Purified recombinant fragment of human TRAF2 (AA: 39-188) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 7186
Other Names TRAP; TRAP3; MGC:45012
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to TRAF2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRAF2
Synonyms TRAP3
Function Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
Cellular Location Cytoplasm
Research Areas
Citations (0)

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References

1.J Virol. 2014 Apr;88(7):3664-77. ; 2.Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Nov;27(11):1176-9.;

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$ 385.00
Cat# AO2340a
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