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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to CD274   

Mouse Monoclonal Antibody to CD274

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to CD274 AO2362a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to CD274 AO2362a
    Western blot analysis using CD274 mAb against human CD274 (AA: 24-153) recombinant protein. (Expected MW is 40.1 kDa)
  • WB - Mouse Monoclonal Antibody to CD274 AO2362a
    Western blot analysis using *** mAb against HEK293 (1) and *** (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to CD274 AO2362a
    Flow cytometric analysis of Hela cells using CD274 mouse mAb (green) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9NZQ7
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7D2A10
Isotype Mouse IgG2b
Calculated MW 33.3kDa
Description This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.;
Immunogen Purified recombinant fragment of human CD274 (AA: 24-153) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 29126
Other Names B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to CD274 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD274 (HGNC:17635)
Function Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).
Cellular Location Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Note=Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. Isoform 2: Endomembrane system; Single-pass type I membrane protein
Tissue Location Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Research Areas
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References

1.Anticancer Res. 2015 Oct;35(10):5369-76. ; 2.Lung Cancer. 2015 Jun;88(3):254-9. ;

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$ 385.00
Cat# AO2362a
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