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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to BIN1   

Mouse Monoclonal Antibody to BIN1

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to BIN1 AO2385a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to BIN1 AO2385a
    Western blot analysis using BIN1 mAb against human BIN1 (AA: 189-398) recombinant protein. (Expected MW is 47.1 kDa)
  • WB - Mouse Monoclonal Antibody to BIN1 AO2385a
    Western blot analysis using BIN1 mAb against HEK293 (1) and BIN1 (AA: 189-398)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to BIN1 AO2385a
    Western blot analysis using BIN1 mouse mAb against C2C12 (1), A431 (2), HEK293 (3), and MCF-7 (4) cell lysate.
  • FC - Mouse Monoclonal Antibody to BIN1 AO2385a
    Flow cytometric analysis of Hela cells using BIN1 mouse mAb (green) and negative control (red).
  • IHC - Mouse Monoclonal Antibody to BIN1 AO2385a
    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BIN1 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession O00499
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 3B6F10
Isotype Mouse IgG2b
Calculated MW 64.7kDa
Description This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.;
Immunogen Purified recombinant fragment of human BIN1 (AA: 189-398) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 274
Other Names AMPH2; AMPHL; SH3P9
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to BIN1 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BIN1
Synonyms AMPHL
Function May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
Cellular Location Isoform BIN1: Nucleus.
Tissue Location Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Research Areas
Citations (0)

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References

1.Trends Mol Med. 2013 Oct;19(10):594-603. ; 2.Mol Med. 2012 May 9;18:507-18. ;

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$ 385.00
Cat# AO2385a
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