|Application ||WB, IHC, FC, E|
|Description||This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.;|
|Immunogen||Purified recombinant fragment of human BIN1 (AA: 189-398) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Application Note||ELISA: 1/10000|
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FCM: 1/200 - 1/400
|Other Names||AMPH2; AMPHL; SH3P9|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Monoclonal Antibody to BIN1 is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.|
|Cellular Location||Isoform BIN1: Nucleus.|
|Tissue Location||Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier Isoform BIN1 is widely expressed with highest expression in skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
1.Trends Mol Med. 2013 Oct;19(10):594-603. ; 2.Mol Med. 2012 May 9;18:507-18. ;
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