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Mouse Monoclonal Antibody to UFD1L

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to UFD1L AO2394a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to UFD1L AO2394a
    Western blot analysis using UFD1L mAb against human UFD1L (AA: 208-307) recombinant protein. (Expected MW is 36.8 kDa)
  • WB - Mouse Monoclonal Antibody to UFD1L AO2394a
    Western blot analysis using UFD1L mAb against HEK293 (1) and UFD1L (AA: 208-307)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to UFD1L AO2394a
    Western blot analysis using UFD1L mouse mAb against K562 (1), Hela (2), A431 (3), PC-2 (4), and A549 (5) cell lysate.
  • FC - Mouse Monoclonal Antibody to UFD1L AO2394a
    Flow cytometric analysis of Hela cells using UFD1L mouse mAb (green) and negative control (red).
  • ICC - Mouse Monoclonal Antibody to UFD1L AO2394a
    Immunofluorescence analysis of Hela cells using UFD1L mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • ICC - Mouse Monoclonal Antibody to UFD1L AO2394a
    Immunofluorescence analysis of Hela cells using UFD1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q92890
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2A6F3
Isotype Mouse IgG2b
Calculated MW 34.5kDa
Description The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.;
Immunogen Purified recombinant fragment of human UFD1L (AA: 208-307) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 7353
Other Names UFD1
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to UFD1L is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UFD1 (HGNC:12520)
Synonyms UFD1L
Function Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (PubMed:26471729).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q9ES53}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9ES53}
Tissue Location Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney
Research Areas
Citations (0)

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References

1.Proc Natl Acad Sci U S A. 2011 May 31;108(22):9119-24. ; 2.Cell Biochem Funct. 2003 Sep;21(3):263-7.V;

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$ 385.00
Cat# AO2394a
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