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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to RBFOX3   

Mouse Monoclonal Antibody to RBFOX3

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to RBFOX3 AO2413a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to RBFOX3 AO2413a
    Western blot analysis using RBFOX3 mAb against human *** (AA: 1-140) recombinant protein. (Expected MW is 41.4 kDa)
  • WB - Mouse Monoclonal Antibody to RBFOX3 AO2413a
    Western blot analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to RBFOX3 AO2413a
    Western blot analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.7 (2), and U251 (3) cell lysate.
  • ICC - Mouse Monoclonal Antibody to RBFOX3 AO2413a
    Immunofluorescence analysis of Hela cells using RBFOX3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • FC - Mouse Monoclonal Antibody to RBFOX3 AO2413a
    Flow cytometric analysis of Hela cells using RBFOX3 mouse mAb (green) and negative control (red).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession A6NFN3
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 5A4B9
Isotype Mouse IgG1
Calculated MW 33.8kDa
Description RBFOX3 (RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3) is a Protein Coding gene. Diseases associated with RBFOX3 include vertebral artery occlusion and tuberculum sellae meningioma. Among its related pathways are Neuroscience. GO annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is RBFOX1.;
Immunogen Purified recombinant fragment of human RBFOX3 (AA: 1-140) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 146713
Other Names FOX3; NEUN; FOX-3; HRNBP3
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to RBFOX3 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RBFOX3
Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Cellular Location Nucleus. Cytoplasm. Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment.
Research Areas
Citations (0)

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References

1.Neurosci Lett. 2014 Jan 13;558:97-102. ; 2.PLoS One. 2013 Sep 6;8(9):e73323. ;

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$ 385.00
Cat# AO2413a
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