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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to SOD2   

Mouse Monoclonal Antibody to SOD2

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to SOD2 AO2423a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to SOD2 AO2423a
    Western blot analysis using SOD2 mAb against human SOD2 (AA: 1-222) recombinant protein. (Expected MW is 50.7 kDa)
  • WB - Mouse Monoclonal Antibody to SOD2 AO2423a
    Western blot analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to SOD2 AO2423a
    Flow cytometric analysis of Hela cells using SOD2 mouse mAb (green) and negative control (red).
  • ICC - Mouse Monoclonal Antibody to SOD2 AO2423a
    Immunofluorescence analysis of Hela cells using SOD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • IHC - Mouse Monoclonal Antibody to SOD2 AO2423a
    Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SOD2 mouse mAb with DAB staining.
  • IHC - Mouse Monoclonal Antibody to SOD2 AO2423a
    Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SOD2 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P04179
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8H3D2
Isotype Mouse IgG1
Calculated MW 24.7kDa
Description This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.;
Immunogen Purified recombinant fragment of human SOD2 (AA: 1-222) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/50 - 1/250
FCM: 1/200 - 1/400
Additional Information
Gene ID 6648
Other Names IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to SOD2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOD2
Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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References

1.Dis Markers. 2015;2015:746329. ; 2.Free Radic Biol Med. 2015 Dec;89:379-86.;

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$ 385.00
Cat# AO2423a
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