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ARFGAP1
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, E |
|---|---|
| Primary Accession | Q8N6T3 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1C4E2 |
| Isotype | Mouse IgG2b |
| Calculated MW | 44.7kDa |
| Immunogen | Purified recombinant fragment of human ARFGAP1 (AA: 270-414) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 55738 |
|---|---|
| Other Names | ARF1GAP; HRIHFB2281 |
| Dilution | E~~ 1/10000 WB~~ 1/500 - 1/2000 FCM~~1/200 - 1/400 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ARFGAP1 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ARFGAP1 |
|---|---|
| Synonyms | ARF1GAP |
| Function | GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity). |
| Cellular Location | Cytoplasm. Golgi apparatus. Note=Associates with the Golgi complex. |
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Provided below are standard protocols that you may find useful for product applications.
References
1.PLoS One. 2014 Nov 14;9(11):e111309. 2.Methods Enzymol. 2001;329:307-16.
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