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>   home   >   Products   >   Primary Antibodies   >   PRMT3 Antibody (C-term)   

PRMT3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PRMT3 Antibody (C-term) AP1005a
    Western blot analysis of anti-PRMT3 Pab (Cat. #AP1005a)in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - PRMT3 Antibody (C-term) AP1005a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IHC-P - PRMT3 Antibody (C-term) AP1005a
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) (Cat.#AP1005a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60678
Other Accession Q922H1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59876 Da
Antigen Region 460-491 aa
Additional Information
Gene ID 10196
Other Names Protein arginine N-methyltransferase 3, 211-, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, PRMT3, HRMT1L3
Target/Specificity This PRMT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 460-491 amino acids from the C-terminal region of human PRMT3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRMT3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRMT3
Synonyms HRMT1L3
Function Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
Cellular Location Cytoplasm.
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Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

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$ 295.00
$ 99.00
Cat# AP1005a
(40 western blots)
Availability: In Stock
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