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C12orf48 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - C12orf48 Antibody (C-term) AP10083b
    C12orf48 Antibody (C-term) (Cat. #AP10083b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C12orf48 antibody detected the FBXO34 protein (arrow).
  • IHC-P - C12orf48 Antibody (C-term) AP10083b
    C12orf48 antibody (C-term) (Cat. #AP10083b) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C12orf48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - C12orf48 Antibody (C-term) AP10083b
    C12orf48 Antibody (C-term) (Cat. #AP10083b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NWS1
Other Accession NP_060385.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 65054 Da
Antigen Region 522-550 aa
Additional Information
Gene ID 55010
Other Names PCNA-interacting partner, PARI, PARP-1 binding protein, PARP1-binding protein, PARPBP, PARPBP, C12orf48, PARI
Target/Specificity This C12orf48 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 522-550 amino acids from the C-terminal region of human C12orf48.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC12orf48 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms C12orf48, PARI
Function Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Binds single-strand DNA and poly(A) homopolymers. Positively regulate the poly(ADP- ribosyl)ation activity of PARP1; however such function may be indirect.
Cellular Location Cytoplasm. Nucleus Note=Localizes to chromatin in response to S phase arrest but not in mitosis. Targeted to chromatin via its interaction with PCNA
Tissue Location Restricted to testis. Overexpressed in multiple cancer cells.
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Cat# AP10083b
(40 western blots)
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