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Protein-C Tag AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1017a | 0.1 mg 400 ul | In Stock | $ 195.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Protein-C Tag Antibody - Product info | |
| Application | WB
|
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | Da |
Protein-C Tag Antibody - Additional info | |
| Target/Specificity KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK) was used as antigen. | |
| Dilution WB~~1:50~100WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Protein-C Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Protein-C Tag Antibody - Related products
Protein-C Tag Antibody - Application data
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Western blot analysis of anti-Protein-C tag Pab (Cat. #AP1017a)in Protein-C-tagged recombinant protein bacterial lysate.
Protein-C Tag Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Abgent's anti-Protein-C polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.
REFERENCES
Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S. Comparison of affinity tags for protein purification. Protein Expr Purif. 2005 May;41(1):98-105.
