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> home > Products > Primary Antibodies > Antibody Collections > Catalog Updated > METTL4 Antibody (C-term)
METTL4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, IHC-P-Leica, E |
|---|---|
| Primary Accession | Q8N3J2 |
| Other Accession | NP_073751.3 |
| Reactivity | Human, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 54041 Da |
| Antigen Region | 315-344 aa |
| Gene ID | 64863 |
|---|---|
| Other Names | Methyltransferase-like protein 4, 211-, METTL4 |
| Target/Specificity | This METTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-344 amino acids from the C-terminal region of human METTL4. |
| Dilution | WB~~1:1000 IHC-P-Leica~~1:500 FC~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | METTL4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | METTL4 {ECO:0000303|PubMed:31913360, ECO:0000312|HGNC:HGNC:24726} |
|---|---|
| Function | N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA (PubMed:31913360, PubMed:32183942). Acts as a N(6)- adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation (PubMed:31913360). Internal m6A(m) methylation of snRNAs regulates RNA splicing (PubMed:31913360). Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)- methyladenosine) (PubMed:32183942). The existence of N(6)- methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)- adenine-specific DNA methyltransferase activity of METTL4 in vivo (PubMed:32203414). Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending (PubMed:32183942). N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (By similarity). |
| Cellular Location | Nucleus. Cytoplasm, cytosol. Mitochondrion matrix |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Trevino, L.R., et al. Nat. Genet. 41(9):1001-1005(2009)
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$ 365.00
$ 140.00
Cat# AP10377B
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