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SCXA Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SCXA Antibody (C-term) AP10439B
    All lanes : Anti-SCXA Antibody (C-term) at 1:1000 dilution Lane 1: HACAT whole cell lysate Lane 2: U-2OS whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - SCXA Antibody (C-term) AP10439B
    Immunohistochemical analysis of AP10439b on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q7RTU7
Other Accession Q64124, P59101, NP_001073983.1, NP_001008272.1
Reactivity Human, Mouse
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 21596 Da
Antigen Region 172-201 aa
Additional Information
Gene ID 642658
Other Names Basic helix-loop-helix transcription factor scleraxis, Class A basic helix-loop-helix protein 41, bHLHa41, Class A basic helix-loop-helix protein 48, bHLHa48, SCX, BHLHA41, BHLHA48, SCXA, SCXB
Target/Specificity This SCXA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-201 amino acids from the C-terminal region of human SCXA.
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCXA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SCX
Synonyms BHLHA41, BHLHA48, SCXA, SCXB
Function Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
Research Areas
Citations ( 0 )

References

Furumatsu, T., et al. Int. J. Biochem. Cell Biol. 42(1):148-156(2010) Liu, Q., et al. Zhonghua Kou Qiang Yi Xue Za Zhi 41(9):556-558(2006) Nusbaum, C., et al. Nature 439(7074):331-335(2006) Schulze-Tanzil, G., et al. Histochem. Cell Biol. 122(3):219-228(2004) Salingcarnboriboon, R., et al. Exp. Cell Res. 287(2):289-300(2003)

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$ 365.00
$ 140.00
Cat# AP10439B
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