|Application ||WB, E|
|Other Accession||Q7M6Z3, Q8R5A0|
|Calculated MW||49688 Da|
|Antigen Region||120-152 aa|
|Other Names||N-lysine methyltransferase SMYD2, 211-, HSKM-B, Histone methyltransferase SMYD2, Lysine N-methyltransferase 3C, SET and MYND domain-containing protein 2, SMYD2, KMT3C|
|Target/Specificity||This SMYD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-152 amino acids from the N-terminal region of human SMYD2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SMYD2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.|
|Cellular Location||Cytoplasm, cytosol. Nucleus|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM].
Abu-Farha,M.,Mol. Cell Proteomics 7 (3), 560-572 (2008)
Ewing,R.M.,Mol. Syst. Biol. 3, 89 (2007)
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