- CITATIONS: 2
|Application ||WB, IHC-P, E|
|Other Accession||P63282, P63281, P63280, P63283, Q9DDJ0, Q9W6H5|
|Predicted||Mouse, Rat, Zebrafish, Chicken, Xenopus|
|Calculated MW||18007 Da|
|Antigen Region||1-30 aa|
|Other Names||SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9|
|Target/Specificity||This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBC9 (UBE2I) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.|
|Cellular Location||Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).|
|Tissue Location||Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.|
Provided below are standard protocols that you may find useful for product applications.
UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.
Biochem Biophys Res Commun. 2002 Aug 30;296(4):870-6.
Genomics. 1996 Oct 15;37(2):183-6.
Cytogenet Cell Genet. 1996;75(4):222-3.
Cytogenet Cell Genet. 1996;72(1):86-9.
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