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HHLA2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HHLA2 Antibody (N-term) AP10650a
    All lanes : Anti-HHLA2 Antibody (N-term) at 1:2000 dilution Lane 1: human pancreas lysate Lane 2: MDA-MB-453 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - HHLA2 Antibody (N-term) AP10650a
    HHLA2 Antibody (N-term) (Cat. #AP10650a) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HHLA2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - HHLA2 Antibody (N-term) AP10650a
    HHLA2 Antibody (N-term) (Cat. #AP10650a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - HHLA2 Antibody (N-term) AP10650a
    HHLA2 Antibody (N-term) (Cat. #AP10650a) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UM44
Other Accession NP_009003.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46850 Da
Antigen Region 58-87 aa
Additional Information
Gene ID 11148
Other Names HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, HHLA2
Target/Specificity This HHLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human HHLA2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHHLA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HHLA2
Function Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, highly expressed in B- cells, dendritic cells and macrophages. Not detected in T-cells
Research Areas
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Mager, D.L., et al. Genomics 59(3):255-263(1999)

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Cat# AP10650a
(40 western blots)
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