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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RICH2 Antibody (C-term)   

RICH2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RICH2 Antibody  (C-term) AP10656B
    RICH2 Antibody (C-term) (Cat. #AP10656b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RICH2 antibody detected the RICH2 protein (arrow).
  • IHC-P - RICH2 Antibody  (C-term) AP10656B
    RICH2 antibody (C-term) (Cat. #AP10656b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RICH2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - RICH2 Antibody  (C-term) AP10656B
    RICH2 Antibody (C-term) (Cat. #AP10656b) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q17R89
Other Accession F1LQX4, NP_055674.4
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 89247 Da
Antigen Region 784-811 aa
Additional Information
Gene ID 9912
Other Names Rho GTPase-activating protein 44, NPC-A-10, Rho-type GTPase-activating protein RICH2, RhoGAP interacting with CIP4 homologs protein 2, RICH-2, ARHGAP44, KIAA0672, RICH2
Target/Specificity This RICH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-811 amino acids from the C-terminal region of human RICH2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRICH2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0672, RICH2
Function GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP- bound states. May act as a GAP for CDC42 and RAC1. Endosomal recycling protein which, in association with SHANK3, is involved in synaptic plasticity. Promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation.
Cellular Location Cell projection, dendritic spine. Recycling endosome. Cell junction, synapse. Note=In CA1 hippocampal synapses, detected at both presynaptic and postsynaptic sites.
Tissue Location Highly expressed in brain. Expressed at weak level in other tissues.
Research Areas
Citations (0)

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GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Acts as a GTPase activitor in vitro for CDC42 and RAC1.


Rollason, R., et al. J. Cell Biol. 184(5):721-736(2009)
Richnau, N., et al. J. Biol. Chem. 276(37):35060-35070(2001)

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Cat# AP10656B
(40 western blots)
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