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C5AR1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - C5AR1 Antibody (Center) AP10701c
    C5AR1 Antibody (Center) (Cat. #AP10701c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C5AR1 antibody detected the C5AR1 protein (arrow).
  • FC - C5AR1 Antibody (Center) AP10701c
    C5AR1 Antibody (Center) (Cat. #AP10701c) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21730
Other Accession NP_001727.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39336 Da
Antigen Region 175-201 aa
Additional Information
Gene ID 728
Other Names C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88, C5AR1, C5AR, C5R1
Target/Specificity This C5AR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human C5AR1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC5AR1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name C5AR1
Synonyms C5AR, C5R1
Function Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed:1847994, PubMed:8182049, PubMed:7622471, PubMed:9553099, PubMed:10636859, PubMed:15153520). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed:8182049, PubMed:7622471, PubMed:9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed:10636859, PubMed:15153520).
Cellular Location Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle. Note=Phosphorylated C5aR colocalizes with ARRB1 and ARRB2 in cytoplasmic vesicles
Research Areas
Citations (0)

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Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.


Skeie, J.M., et al. Invest. Ophthalmol. Vis. Sci. 51(10):5336-5342(2010)
Nishiura, H., et al. Apoptosis 15(8):966-981(2010)
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Marc, M.M., et al. Scand. J. Immunol. 71(5):386-391(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)

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Cat# AP10701c
(40 western blots)
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