|Application ||IHC-P, WB, E|
|Other Accession||O77768, Q9Z204, NP_004491.2|
|Calculated MW||33670 Da|
|Antigen Region||279-306 aa|
|Other Names||Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2, HNRNPC, HNRPC|
|Target/Specificity||This HNRNPC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-306 amino acids from the C-terminal region of human HNRNPC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HNRNPC Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6- methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non- coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre- mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'- UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).|
|Cellular Location||Nucleus. Note=Component of ribonucleosomes.|
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Provided below are standard protocols that you may find useful for product applications.
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq].
Konig, J., et al. Nat. Struct. Mol. Biol. 17(7):909-915(2010)
Lee, E.K., et al. Nat. Struct. Mol. Biol. 17(6):732-739(2010)
Brunner, J.E., et al. Virology 400(2):240-247(2010)
Ertel, K.J., et al. J. Virol. 84(9):4229-4242(2010)
Mosessian, S., et al. J. Biol. Chem. 284(44):30159-30166(2009)
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