|Application ||WB, FC, E|
|Other Accession||Q99N37, Q3UIA2, NP_001006635.1|
|Calculated MW||95437 Da|
|Antigen Region||41-70 aa|
|Other Names||Rho GTPase-activating protein 17, Rho-type GTPase-activating protein 17, RhoGAP interacting with CIP4 homologs protein 1, RICH-1, ARHGAP17, RICH1|
|Target/Specificity||This ARHGAP17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human ARHGAP17.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARHGAP17 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.|
|Cellular Location||Membrane; Peripheral membrane protein. Cytoplasm. Cell junction, tight junction. Note=Associates with membranes and concentrates at sites of cell-cell contact|
|Tissue Location||Ubiquitously expressed. Expressed at higher level in heart and placenta.|
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Provided below are standard protocols that you may find useful for product applications.
RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA (MIM 165390), RAC1 (MIM 602048), and CDC42 (MIM 116952).[supplied by OMIM].
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Wells, C.D., et al. Cell 125(3):535-548(2006)
Richnau, N., et al. J. Biol. Chem. 276(37):35060-35070(2001)
Reczek, D., et al. J. Cell Biol. 153(1):191-206(2001)
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