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CWC22 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | Q9HCG8 |
| Other Accession | NP_065994.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 105466 Da |
| Antigen Region | 16-45 aa |
| Gene ID | 57703 |
|---|---|
| Other Names | Pre-mRNA-splicing factor CWC22 homolog, Nucampholin homolog, fSAPb, CWC22, KIAA1604, NCM |
| Target/Specificity | This CWC22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human CWC22. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CWC22 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CWC22 |
|---|---|
| Synonyms | KIAA1604, NCM |
| Function | Required for pre-mRNA splicing as component of the spliceosome (PubMed:12226669, PubMed:11991638, PubMed:22961380, PubMed:28502770, PubMed:28076346, PubMed:29360106, PubMed:29301961). As a component of the minor spliceosome, involved in the splicing of U12- type introns in pre-mRNAs (Probable). Promotes exon-junction complex (EJC) assembly (PubMed:22959432, PubMed:22961380). Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay. |
| Cellular Location | Nucleus. Nucleus speckle. Note=Concentrates around speckles, which are sites of pre-mRNA synthesis and processing, where it colocalizes with EJC core proteins. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
May be involved in pre-mRNA splicing.
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Jurica, M.S., et al. RNA 8(4):426-439(2002)
Kelly, W.G., et al. Genetics 146(1):227-238(1997)
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