|Application ||WB, IHC-P, FC, E|
|Calculated MW||105466 Da|
|Antigen Region||16-45 aa|
|Other Names||Pre-mRNA-splicing factor CWC22 homolog, Nucampholin homolog, fSAPb, CWC22, KIAA1604, NCM|
|Target/Specificity||This CWC22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human CWC22.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CWC22 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for pre-mRNA splicing and for exon-junction complex (EJC) assembly. Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay.|
|Cellular Location||Nucleus. Nucleus speckle. Note=Concentrates around speckles, which are sites of pre-mRNA synthesis and processing, where it colocalizes with EJC core proteins|
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Provided below are standard protocols that you may find useful for product applications.
May be involved in pre-mRNA splicing.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Jurica, M.S., et al. RNA 8(4):426-439(2002)
Kelly, W.G., et al. Genetics 146(1):227-238(1997)
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