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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RN185 Antibody (Center)   

RN185 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RN185 Antibody  (Center) AP10818c
    RN185 Antibody (Center) (Cat. #AP10818c) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).
  • FC - RN185 Antibody  (Center) AP10818c
    RN185 Antibody (Center) (Cat. #AP10818c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • WB - RN185 Antibody  (Center) AP10818c
    RN185 Antibody (Center) (Cat. #AP10818c) western blot analysis in mouse kidney tissue and K562 cell line lysates (35ug/lane).This demonstrates the RN185 antibody detected the RN185 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96GF1
Other Accession Q568Y3, Q91YT2, Q6PC78, Q5ZIR9, NP_689480.2
Reactivity Human, Mouse, Hamster
Predicted Rat, Zebrafish, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 20459 Da
Antigen Region 88-117 aa
Additional Information
Gene ID 91445
Other Names E3 ubiquitin-protein ligase RNF185, 632-, RING finger protein 185, RNF185
Target/Specificity This RN185 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human RN185.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRN185 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF185
Function E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein
Research Areas
Citations ( 0 )


Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :

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$ 295.00
$ 99.00
Cat# AP10818c
(40 western blots)
Availability: In Stock
Bulk Size
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