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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VPS52 Antibody (C-term)   

VPS52 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - VPS52 Antibody (C-term) AP10925b
    VPS52 Antibody (C-term) (Cat. #AP10925b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the VPS52 antibody detected the VPS52 protein (arrow).
  • IHC-P - VPS52 Antibody (C-term) AP10925b
    VPS52 antibody (C-term) (Cat. #AP10925b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VPS52 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - VPS52 Antibody (C-term) AP10925b
    VPS52 Antibody (C-term) (Cat. #AP10925b) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - VPS52 Antibody (C-term) AP10925b
    Confocal immunofluorescent analysis of VPS52 Antibody (C-term)(Cat#AP10925b) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8N1B4
Other Accession O55166, Q8C754, NP_072047.4
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 82221 Da
Antigen Region 610-637 aa
Additional Information
Gene ID 6293
Other Names Vacuolar protein sorting-associated protein 52 homolog, SAC2 suppressor of actin mutations 2-like protein, VPS52, SACM2L
Target/Specificity This VPS52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-637 amino acids from the C-terminal region of human VPS52.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVPS52 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS52
Synonyms SACM2L
Function May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.
Cellular Location Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein
Research Areas
Citations (0)

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This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.


Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) : Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009) Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008) Liewen, H., et al. Exp. Cell Res. 306(1):24-34(2005) Mungall, A.J., et al. Nature 425(6960):805-811(2003)

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Cat# AP10925b
(40 western blots)
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