|Application ||WB, FC, E|
|Calculated MW||129242 Da|
|Antigen Region||293-322 aa|
|Other Names||Phospholipid-transporting ATPase IB, ATPase class I type 8A member 2, ML-1, P4-ATPase flippase complex alpha subunit ATP8A2, ATP8A2, ATPIB|
|Target/Specificity||This ATP8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from the N-terminal region of human ATP8A2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP8A2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Reconstituted to liposomes, the ATP8A2:TMEM30A flippase complex predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). Proposed to function in the generation and maintenance of phospholipid asymmetry in photoreceptor disk membranes and neuronal axon membranes. May be involved in vesicle trafficking in neuronal cells. Involved in regulation of neurite outgrowth; acting in synergy with TMEM30A. Required for normal visual and auditory function; involved in photoreceptor and inner ear spiral ganglion cell survival.|
|Cellular Location||Membrane; Multi-pass membrane protein. Golgi apparatus. Endosome. Cell projection, cilium, photoreceptor outer segment Cell membrane. Note=Localizes to the Golgi and endosomes in photoreceptor cells. Localizes to disk membranes of rod photoreceptor outer segments (ROS) (By similarity).|
|Tissue Location||Strongly expressed in the brain, cerebellum, retina and testis.|
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Provided below are standard protocols that you may find useful for product applications.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Coleman, J.A., et al. J. Biol. Chem. 284(47):32670-32679(2009)
Dunham, A., et al. Nature 428(6982):522-528(2004)
Halleck, M.S., et al. Physiol. Genomics 1(3):139-150(1999)
Sun, X.L., et al. Gene Expr. 8(2):129-139(1999)
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