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>   home   >   Products   >   Primary Antibodies   >   ATP8A2 Antibody (N-term)   

ATP8A2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP8A2 Antibody (N-term) AP10934a
    ATP8A2 Antibody (N-term) (Cat. #AP10934a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ATP8A2 antibody detected the ATP8A2 protein (arrow).
  • FC - ATP8A2 Antibody (N-term) AP10934a
    ATP8A2 Antibody (N-term) (Cat. #AP10934a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NTI2
Other Accession NP_057613.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 129242 Da
Antigen Region 293-322 aa
Additional Information
Gene ID 51761
Other Names Phospholipid-transporting ATPase IB, ATPase class I type 8A member 2, ML-1, P4-ATPase flippase complex alpha subunit ATP8A2, ATP8A2, ATPIB
Target/Specificity This ATP8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from the N-terminal region of human ATP8A2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP8A2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP8A2
Synonyms ATPIB
Function Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Reconstituted to liposomes, the ATP8A2:TMEM30A flippase complex predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). Proposed to function in the generation and maintenance of phospholipid asymmetry in photoreceptor disk membranes and neuronal axon membranes. May be involved in vesicle trafficking in neuronal cells. Involved in regulation of neurite outgrowth; acting in synergy with TMEM30A. Required for normal visual and auditory function; involved in photoreceptor and inner ear spiral ganglion cell survival.
Cellular Location Membrane; Multi-pass membrane protein. Golgi apparatus. Endosome. Cell projection, cilium, photoreceptor outer segment Cell membrane. Note=Localizes to the Golgi and endosomes in photoreceptor cells. Localizes to disk membranes of rod photoreceptor outer segments (ROS) (By similarity).
Tissue Location Strongly expressed in the brain, cerebellum, retina and testis.
Citations (0)

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Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Coleman, J.A., et al. J. Biol. Chem. 284(47):32670-32679(2009)
Dunham, A., et al. Nature 428(6982):522-528(2004)
Halleck, M.S., et al. Physiol. Genomics 1(3):139-150(1999)
Sun, X.L., et al. Gene Expr. 8(2):129-139(1999)

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$ 295.00
$ 99.00
Cat# AP10934a
(40 western blots)
Availability: In Stock
Bulk Size
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