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IGHG1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IGHG1 Antibody (Center) AP10959c
    Anti-IGHG1 Antibody (Center) at 1:2000 dilution + human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IGHG1 Antibody (Center) AP10959c
    All lanes : Anti-IGHG1 Antibody (Center) at 1:2000 dilution Lane 1: human fetal spleen lysate Lane 2: human fetal thymus lysate Lane 3: human spleen lysate Lane 4: human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • FC - IGHG1 Antibody (Center) AP10959c
    Overlay histogram showing HL-60 cells stained with AP10959c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10959c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01857
Other Accession P01860
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36106 Da
Antigen Region 154-180 aa
Additional Information
Other Names Ig gamma-1 chain C region, IGHG1
Target/Specificity This IGHG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-180 amino acids from the Central region of human IGHG1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGHG1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGHG1
Cellular Location Secreted.
Research Areas
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$ 295.00
$ 99.00
Cat# AP10959c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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Ordering Information

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