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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GCNT3 Antibody (C-term)   

GCNT3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GCNT3 Antibody (C-term) AP10975b
    GCNT3 Antibody (C-term) (Cat. #AP10975b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the GCNT3 antibody detected the GCNT3 protein (arrow).
  • IHC-P - GCNT3 Antibody (C-term) AP10975b
    GCNT3 antibody (C-term) (Cat. #AP10975b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCNT3 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - GCNT3 Antibody (C-term) AP10975b
    GCNT3 Antibody (C-term) (Cat. #AP10975b) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95395
Other Accession Q8CH87, Q5JCT0, NP_004742.1
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50864 Da
Antigen Region 390-418 aa
Additional Information
Gene ID 9245
Other Names Beta-1, 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase 3, C2GnT-mucin type, C2GnT-M, hC2GnT-M, Core 2/core 4 beta-1, 6-N-acetylglucosaminyltransferase, C2/4GnT, GCNT3
Target/Specificity This GCNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-418 amino acids from the C-terminal region of human GCNT3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGCNT3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GCNT3
Function Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.
Research Areas
Citations (0)

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This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.


Tan, S., et al. Am. J. Respir. Cell Mol. Biol. 36(6):737-745(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Huang, M.C., et al. Oncogene 25(23):3267-3276(2006)
Beum, P.V., et al. Am. J. Respir. Cell Mol. Biol. 29(1):48-56(2003)
Schwientek, T., et al. J. Biol. Chem. 274(8):4504-4512(1999)

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Cat# AP10975b
(40 western blots)
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