|Application ||WB, IHC-P, FC, E|
|Other Accession||Q8BX80, NP_001036038.1|
|Calculated MW||83987 Da|
|Antigen Region||326-354 aa|
|Other Names||Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE|
|Target/Specificity||This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ENASE Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.|
|Cellular Location||Cytoplasm, cytosol.|
|Tissue Location||Widely expressed. Expressed at higher level in thymus and spleen.|
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Provided below are standard protocols that you may find useful for product applications.
Endo-beta-N-acetylglucosaminidase (ENGase; EC 126.96.36.199) is involved in the processing of free oligosaccharides in the cytosol.
Park, K., et al. Diabetes 59(7):1845-1850(2010)
Dorfmueller, H.C., et al. FEBS Lett. 584(4):694-700(2010)
Chantret, I., et al. PLoS ONE 5 (7), E11734 (2010) :
Butkinaree, C., et al. J. Biol. Chem. 283(35):23557-23566(2008)
Ortutay, Z., et al. Arthritis Rheum. 48(8):2163-2172(2003)
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