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RARS Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP11091b 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP11091b-ev20 20 ug 100 ul In Stock $ 95.00 Add to cart
  • Specification
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  • Backgrounds

RARS Antibody (C-term) - Product info

ApplicationWB, IHC, IF, FC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP54136
Other AccessionNP_002878.2
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW75379 Da

RARS Antibody (C-term) - Additional info

Gene ID 5917
Other Names
RARS; Arginine--tRNA ligase, cytoplasmic; Arginyl-tRNA synthetase
Target/Specificity
This RARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 612-642 amino acids from the C-terminal region of human RARS.
Dilution
WB~~1:50~100
IHC~~1:50~100
IF~~1:10~50
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
RARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

RARS Antibody (C-term) - Protein Information

Name RARS
Function
Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1
Cellular Location
Cytoplasm.

RARS Antibody (C-term) - Related products

AP11091b: RARS Antibody (C-term)

AP7988c: RARS Antibody (Center)

RI14456: RARS predesign siRNA

DC00252: Human RARS cDNA Clone

LY13757a: RARS Over-expression Lysate

AT3573a: RARS Antibody (monoclonal) (M01)

AF2229a: RARS Antibody

RARS Antibody (C-term) - Application data

  • RARS Antibody (C-term) (Cat. #AP11091b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RARS antibody detected the RARS protein (arrow).

  • RARS Antibody (C-term) (Cat. #AP11091b)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

  • Confocal immunofluorescent analysis of RARS Antibody (C-term)(Cat#AP11091b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

  • RARS Antibody (C-term) (Cat. #AP11091b) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

RARS Antibody (C-term) - Research Areas

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BACKGROUND

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq].

REFERENCES

Bottoni, A., et al. J. Cell. Physiol. 212(2):293-297(2007) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005) Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003) Kaminska, M., et al. Biochemistry 40(47):14309-14316(2001)