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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KARS Antibody (N-term)   

KARS Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - KARS Antibody (N-term) AP11097a
    KARS Antibody (N-term) (Cat. #AP11097a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the KARS antibody detected the KARS protein (arrow).
  • WB - KARS Antibody (N-term) AP11097a
    Western blot analysis of KARS (arrow) using rabbit polyclonal KARS Antibody (N-term) (Cat. #AP11097a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.
  • FC - KARS Antibody (N-term) AP11097a
    KARS Antibody (N-term) (Cat. #AP11097a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15046
Other Accession NP_005539.1, NP_001123561.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68048 Da
Antigen Region 70-98 aa
Additional Information
Gene ID 3735
Other Names Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070
Target/Specificity This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human KARS.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0070
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Cellular Location Isoform Cytoplasmic: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted Note=Secretion is induced by TNF-alpha
Research Areas
Citations (0)

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Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.


McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010)
Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010)
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Kawamata, H., et al. J. Biol. Chem. 283(42):28321-28328(2008)

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$ 295.00
$ 99.00
Cat# AP11097a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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