|Application ||WB, FC, E|
|Other Accession||NP_005539.1, NP_001123561.1|
|Calculated MW||68048 Da|
|Antigen Region||70-98 aa|
|Other Names||Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070|
|Target/Specificity||This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human KARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.|
|Cellular Location||Isoform Cytoplasmic: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted Note=Secretion is induced by TNF-alpha|
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Provided below are standard protocols that you may find useful for product applications.
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010)
Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010)
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Kawamata, H., et al. J. Biol. Chem. 283(42):28321-28328(2008)
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