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KARS Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP11097a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP11097a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
KARS Antibody (N-term) - Product info | |
| Application | WB, FC
|
| Primary Accession | Q15046 |
| Other Accession | NP_005539.1, NP_001123561.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 68048 Da |
KARS Antibody (N-term) - Additional info | |
| Gene ID 3735 | |
| Other Names KARS; KIAA0070; Lysine--tRNA ligase; Lysyl-tRNA synthetase | |
| Target/Specificity This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human KARS. | |
| Dilution WB~~1:50~100WB~~1:1000 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions KARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
KARS Antibody (N-term) - Protein Information | |
| Name KARS | |
| Synonyms KIAA0070 | |
| Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation | |
| Cellular Location Isoform Cytoplasmic: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted Note=Secretion is induced by TNF-alpha | |
KARS Antibody (N-term) - Related products
AP11097a: KARS Antibody (N-term)
AP7833b: KARS (Lysyl-tRNA synthetase) Antibody (C-term)
KARS Antibody (N-term) - Application data
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KARS Antibody (N-term) (Cat. #AP11097a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the KARS antibody detected the KARS protein (arrow).
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Western blot analysis of KARS (arrow) using rabbit polyclonal KARS Antibody (N-term) (Cat. #AP11097a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.
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KARS Antibody (N-term) (Cat. #AP11097a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
KARS Antibody (N-term) - Research Areas
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BACKGROUND
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
REFERENCES
McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010) Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010) Segat, L., et al. Vaccine 28(10):2201-2206(2010) Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010) Kawamata, H., et al. J. Biol. Chem. 283(42):28321-28328(2008)
