|Application ||WB, FC, E|
|Calculated MW||33243 Da|
|Antigen Region||1-30 aa|
|Other Names||Cyclin-C, SRB11 homolog, hSRB11, CCNC|
|Target/Specificity||This CCNC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CCNC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CCNC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.|
|Tissue Location||Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.
Miyata, Y., et al. Stem Cells 28(2):308-317(2010)
Makkonen, K.M., et al. J. Mol. Biol. 393(2):261-271(2009)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Katona, R.L., et al. Acta. Biol. Hung. 58(1):133-137(2007)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
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