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PLD6 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PLD6 Antibody (Center) AP11286c
    Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PLD6 Antibody (Center) AP11286c
    Anti-PLD6 Antibody (Center) at 1:2000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PLD6 Antibody (Center) AP11286c
    All lanes : Anti-PLD6 Antibody (Center) at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PLD6 Antibody (Center) AP11286c
    Western blot analysis of lysates from human ovary and mouse testis tissue lysates (from left to right), using PLD6 Antibody (Center)(Cat. #AP11286c). AP11286c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - PLD6 Antibody (Center) AP11286c
    PLD6 Antibody (Center) (Cat. #AP11286c) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8N2A8
Other Accession NP_849158.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28273 Da
Antigen Region 125-154 aa
Additional Information
Gene ID 201164
Other Names Mitochondrial cardiolipin hydrolase, 31--, Choline phosphatase 6, Mitochondrial phospholipase, MitoPLD, Phosphatidylcholine-hydrolyzing phospholipase D6, Phospholipase D6, PLD 6, Protein zucchini homolog, PLD6
Target/Specificity This PLD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human PLD6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLD6 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLD6
Function Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol.
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein
Tissue Location Widely expressed. Expression is not limited to testis and ovary.
Research Areas
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$ 295.00
$ 99.00
Cat# AP11286c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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