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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PRMT4 Antibody(Center)   

PRMT4 Antibody(Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PRMT4 Antibody(Center) AP11313c
    PRMT4 Antibody(Center) (Cat. #AP11313c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PRMT4 antibody detected the PRMT4 protein (arrow).
  • IHC-P - PRMT4 Antibody(Center) AP11313c
    PRMT4 Antibody (Center) (Cat. #AP11313c)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PRMT4 Antibody(Center) AP11313c
    PRMT4 Antibody(Center) (Cat. #AP11313c) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86X55
Other Accession Q4AE70, Q9WVG6, NP_954592.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 65854 Da
Antigen Region 346-377 aa
Additional Information
Gene ID 10498
Other Names Histone-arginine methyltransferase CARM1, 211-, Coactivator-associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4, CARM1, PRMT4
Target/Specificity This PRMT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-377 amino acids from the Central region of human PRMT4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRMT4 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CARM1
Synonyms PRMT4
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs.
Cellular Location Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane
Tissue Location Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia
Research Areas
Citations (0)

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Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).


Gao, X., et al. J. Cell. Biochem. 110(1):162-170(2010)
Carascossa, S., et al. Genes Dev. 24(7):708-719(2010)
Kim, Y.R., et al. BMC Cancer 10, 197 (2010) :
Ito, T., et al. BMC Dev. Biol. 9, 47 (2009) :
Haiman, C.A., et al. BMC Cancer 9, 43 (2009) :

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Cat# AP11313c
(40 western blots)
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