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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AP1M1 Antibody (Center)   

AP1M1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AP1M1 Antibody (Center) AP11351c
    All lanes : Anti-AP1M1 Antibody (Center) at 1:1000 dilution Lane 1: Human brain lysate Lane 2: PC-3 whole cell lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AP1M1 Antibody (Center) AP11351c
    AP1M1 Antibody (Center) (Cat. #AP11351c) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the AP1M1 antibody detected the AP1M1 protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BXS5
Other Accession Q32Q06, P35585, Q2KJ81, NP_115882.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48587 Da
Antigen Region 205-234 aa
Additional Information
Gene ID 8907
Other Names AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin, AP1M1, CLTNM
Target/Specificity This AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-234 amino acids from the Central region of human AP1M1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAP1M1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AP1M1
Synonyms CLTNM
Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Cellular Location Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex
Research Areas
Citations (0)

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Background

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].

References

Sawasdee, N., et al. Biochem. Biophys. Res. Commun. 401(1):85-91(2010)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Noviello, C.M., et al. J. Virol. 82(3):1249-1258(2008)
Medigeshi, G.R., et al. Traffic 9(1):121-132(2008)
Roeth, J.F., et al. J. Cell Biol. 167(5):903-913(2004)

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$ 295.00
$ 99.00
Cat# AP11351c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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